Centers
Sequencing & physical mapping center
Sequences are analyzed by AGI Sequencing Center using two ABI 3730 DNA Analyzers. Custom and project small insert genomic (shotgun) libraries are produced by complete or partial restriction enzyme digestion, hydroshear, or sonicator (shatter) into a variety of vectors. AGI Resource Center provides support for robotic picking, gridding and colony handling using Genetix Q-bots.The AGI Physical Mapping Center conducts analysis of BAC libraries by means of high throughput BAC DNA fingerprinting using HICF SNaPshot labeling chemistry. The resulting fingerprints can be assembled by the program FPC to create contiguous clone maps which, in turn provide the basis for development of physical DNA maps. Daily capacity HICF SNaPshot samples is 9- 384-well plates per day, using one AB 3730xl DNA analyzer. Fluorescent trace data is analyzed using AB software (Genemapper) and in-house scripts.
