Arizona Genomics Institute

Leader of Physical Mapping Center:

• Dave Kudrna

Project Leader for Maize Physical Map Analysis:

• Dr. Fusheng Wei

Senior Research Specialist:

• Jose Luis Goicoechea

The AGI Physical Mapping Center conducts analysis of BAC libraries by means of high throughput BAC DNA fingerprinting using HICF SNaPshot labeling chemistry. The resulting fingerprints can be assembled by the program FPC to create contiguous clone maps which, in turn provide the basis for development of physical DNA maps. Daily capacity HICF SNaPshot samples is 9- 384-well plates per day, using one AB 3730xl DNA analyzer. Fluorescent trace data is analyzed using AB software (Genemapper) and in-house scripts.
All fingerprints are assembled into contigs using Fingerprint Contig software (FPC) developed by Dr. Cari Soderlund at AGCoL.

Recent research projects include:

• 10x genome coverage of a representative species from each genome from the Oryza genus for the objectives of the NSF project called "Oryza Map Alignment Project" (www.omap.org).
• High Information Content physical map of the maize genome in collaboration with J. Messing of Rutgers University (NSF #0211851) ;
• 14X map of maize as part of the Maize Mapping Project (MMP, NSF #9872655 );
• An agarose-based physical map of the tomato genome.